Database of Maize Genes with Fluorescent Tags

BLAST server

A set of maize genes was selected for fluorescent tagging (FP) and the generation of reporter lines. Out of the selected maize genes, a total of 85 genes were mapped to the B73 maize genome (www.maizesequence.org). Gene annotation of the native gene models (without FP tag) were downloaded for the following BLAST databases.

BLAST databases:

Protein sequences for tagged maize genes - aa (protein translation)
Coding sequences for tagged maize genes - cds (coding sequence including start to stop)
Genomic sequences for tagged maize genes - genic (genic region including 4 kb upstream and 4 kb downstream flanking sequences)

Using the BLAST server, users can search the maize gene set used for FP-tagging to identify genes of interest based on sequence similarility.

This server runs WU-BLAST 2.0. [Disclaimer]

Select a program and database to search against:

Program:

Database:

Alignments:

Upload a file containing a sequence OR paste it into the textbox:

(Note: If both are entered, the file will be ignored.)

Enter the name of the file containing a sequence in FASTA or raw format:

Enter your sequence in FASTA or raw format:

Sequence identifier (for definition line if raw sequence entered):

Options:

Matrix:	Filter:	Expect:
Cutoff:	Strand:	Descriptions:
Wordlength (for blastn only):	Echofilter	Ignore Hypotheticals
Graphical Overview	Map Overview	PID (map overview only)

The email option allows the user to be notified by email when the blast search is completed. The email will contain a link to the results. Use this option when running a lengthy search. To activate this option, check the box to the left and fill in an email address in the box to the right.
Send a link to results to email address: