Publications

Citation

Martin-Gallardo, A., McCombie, W. R., Gocayne, J. D., FitzGerald, M. G., Wallace, S., Lee, B. M., Lamerdin, J., Trapp, S., Kelley, J. M., Liu, L. I., Dubnick, M., Johnston-Dow, L. A., Kerlavage, A. R., de Jong, P., Carrano, A., Fields, C., Venter, J. C.

Automated DNA Sequencing and Analysis of 106 Kilobases from Human chromosome 19q13.3

Nat Genet. 1992 Apr 01; 1(1): 34-9.

PubMed Citation

Abstract

A total of 116,118 basepairs (bp) derived from three cosmids spanning the ERCC1 locus of human chromosome 19q13.3 have been sequenced with automated fluorescence-based sequencers and analysed by polymerase chain reaction amplification and computer methods. The assembled sequence forms two contigs totalling 105,831 bp, which contain a human fosB proto-oncogene, a gene encoding a protein phosphatase, two genes of unknown function and the previously-characterized ERCC1 DNA repair gene. This light band region has a high average density of 1.4 Alu repeats per kilobase. Human chromosome light bands could therefore contain up to 75,000 genes and 1.5 million Alu repeats.