Publications
Citation
Gibson, D. G., Smith, H. O., Hutchison, C. A., 3rd, Venter, J. C., Merryman, C.
Chemical Synthesis of the Mouse Mitochondrial Genome
Nat Methods. 2010 Oct 10;
Abstract
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.