JCVI: FR-HIT, a Very Fast Program to Recruit Metagenomic Reads to Homologous Reference Genomes.
 
 
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Citation

Niu B, Zhu Z, Fu L, Wu S, Li W

FR-HIT, a Very Fast Program to Recruit Metagenomic Reads to Homologous Reference Genomes.

Bioinformatics (Oxford, England). 2011 Jun 15; 27: 1704-5.

External Citation

Abstract

Fragment recruitment, a process of aligning sequencing reads to reference genomes, is a crucial step in metagenomic data analysis. The available sequence alignment programs are either slow or insufficient for recruiting metagenomic reads. We implemented an efficient algorithm, FR-HIT, for fragment recruitment. We applied FR-HIT and several other tools including BLASTN, MegaBLAST, BLAT, LAST, SSAHA2, SOAP2, BWA and BWA-SW to recruit four metagenomic datasets from different type of sequencers. On average, FR-HIT and BLASTN recruited significantly more reads than other programs, while FR-HIT is about two orders of magnitude faster than BLASTN. FR-HIT is slower than the fastest SOAP2, BWA and BWA-SW, but it recruited 1-5 times more reads.

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