Publications
Citation
Biancucci M, Dolores JS, Wong J, Grimshaw S, Anderson WF, Satchell KJ, Kwon K
New Ligation Independent Cloning Vectors for Expression of Recombinant Proteins With a Self-cleaving CPD/6xHis-tag.
BMC Biotechnology. 2017 Jan 05; 17: 1.
Abstract
Recombinant protein purification is a crucial step for biochemistry and structural biology fields. Rapid robust purification methods utilize various peptide or protein tags fused to the target protein for affinity purification using corresponding matrices and to enhance solubility. However, affinity/solubility-tags often need to be removed in order to conduct functional and structural studies, adding complexities to purification protocols.