Publications
Citation
Hansen, A., Chen, Y., Inman, J. M., Phan, Q. N., Qi, Z. Q., Xiang, C. C., Palkovits, M., Cherman, N., Kuznetsov, S. A., Robey, P. G., Mezey, E., Brownstein, M. J.
Sensitive and Specific Method for Detecting G Protein-coupled Receptor MRNAs
Nat Methods. 2007 Jan 01; 4(1): 35-7.
Abstract
G protein-coupled receptors (GPCRs) mediate effects of extracellular signaling molecules in all the body's cells. These receptors are encoded by scarce mRNAs; therefore, detecting their transcripts with conventional microarrays is difficult. We present a method based on multiplex PCR and array detection of amplicons to assay GPCR gene expression with as little as 1 mug of total RNA, and using it, we profiled three human bone marrow stromal cell (BMSC) lines.