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Proteomics

The PFGRC Proteomics Facility couples sophisticated protein separation technologies with electrospray and MALDI mass spectrometry to conduct research studies and develop publicly available data sets focused on microbial agents of infectious disease and biodefense. Studies conducted thus far have included a comparative protein abundance analysis of three isogenic Staphylococcus aureus strains derived from a clinical vancomcyin-intermediate S. aureus (VISA) isolate (in collaboration with Emmanuel Mongodin, the J. Craig Venter Institute and Steve Gill, SUNY, Buffalo), the characterization of the periplasmic subcellular proteome of the Yersinia pestis strain KIM6+ in defined medium in vitro and in response to temperature shifts (26°C and 37°C) (in collaboration with Robert Perry, University of Kentucky), and a comparison of in vitro and in vivo proteome profiles, including membrane associated proteins of the enteropathogenic bacterium Shigella dysenteriae (in collaboration with Saul Tzipori, Tufts Cummings School of Veterinary Medicine).

In addition to proteome profiling, the PFGRC Proteomics Group is developing high throughput platforms for in vitro and in vivo protein expression and purification utilizing the Gateway® Destination Vector System.  Proteins expressed and purified in this manner will be targeted for specific applications utilizing protein microarrays.

The table below provides links to proteome data sets relevant to each of the studies described above.

Project Description / Publications

Reagents and Resources

Collaborators

Proteomic Profiling of Cell Envelope-Associated Proteins from Staphylococcus aureus

Data Presentation Resource

Emmanuel Mongodin, Steve Gill (the J. Craig Venter Institute)

Comparative Proteomic Analysis of Staphylococcus aureus Strains with Differences in Resistance to the Cell Wall-Targeting Antibiotic Vancomycin

Data Presentation Resource

Emmanuel Mongodin, Steve Gill (the J. Craig Venter Institute)

Proteomic analysis of enterohemorrhagic E. coli (EHEC strain O157:H7) isolated from the infected piglet gut and in vitro cultured cells

Data Presentation Resource

Dr. Saul Tzipori, Dr. Arthur Donohue-Rolfe, Dr. Quanshun Zhang (Division of Infectious Diseases, Cummings School of Veterinary Medicine, Tufts University)

The Shigella dysenteriae serotype 1 proteome, profiled in the host intestinal environment, reveals major metabolic modifications and increased expression of invasive proteins

Data Presentation Resource

Dr. Saul Tzipori, Dr. Arthur Donohue-Rolfe, Dr. Quanshun Zhang (Division of Infectious Diseases, Cummings School of Veterinary Medicine, Tufts University)

Comparison of two label-free global quantitation methods, APEX and 2D gel electrophoresis, applied to the Shigella dysenteriae proteome

Data Presentation Resource

Dr. Saul Tzipori, Dr. Arthur Donohue-Rolfe, Dr. Quanshun Zhang (Division of Infectious Diseases, Cummings School of Veterinary Medicine, Tufts University)

Widespread Occurrence of Non-Enzymatic Deamidations of Asparagine Residues in Yersinia pestis Proteins Resulting from Alkaline pH Membrane Extraction Conditions

Data Presentation Resource

Integral and peripheral association of proteins and protein complexes with Yersinia pestis inner and outer membranes

Data Presentation Resource

Dr. Robert Perry (Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky)

Characterizing the dynamic nature of the Yersinia pestis periplasmic proteome in response to nutrient exhaustion and temperature change

Data Presentation Resource

Dr. Robert Perry (Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky)

Temperature and Growth Phase Influence the Outer Membrane Proteome and the Expression of a Type VI Secretion System in Yersinia pestis

Data Presentation Resource

Dr. Robert Perry (Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky)

A correlation analysis of protein characteristics associated with genome-wide high throughput expression and solubility of Streptococcus pneumoniae proteins

Data Presentation Resource

High quality protein microarray using in situ protein purification

Data Presentation Resource

Using chemical derivatization and mass spectrometric analysis to characterize the post-translationally modified Staphylococcus aureus surface protein G

Data Presentation Resource

Proteomic analysis of iron acquisition, metabolic and regulatory responses of Yersinia pestis to iron starvation

Data Presentation Resource

Dr. Robert Perry (Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky)

Identification of cell wall and cytoplasmic proteins of Aspergillus fumigatus

Data Presentation Resource

Dr. Michelle Momany (Department of Plant Biology, University of Georgia)

Poster Presentations

Presentation Resource

PFGRC - Pathogen Functional Genomics Resource Center

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